Preventive Effects of NSAIDs on Lung Tissue Oxidative Damage in an Animal Sepsis Model

Background: Sepsis is a heterogeneous syndrome caused by a dysregulated host response to infection. Inflammatory cascades have a critical role in sepsis and can potentially be suppressed by anti-inflammatory compounds. Therefore, this study was focused on the antiseptic effects of non-steroidal anti-inflammatory drugs (NSAIDs) on lung injuries based on cecal ligation and puncture (CLP) surgery. Materials and Methods: Male Wistar rats were divided into ۶ groups (n=۶۰) as follows: Control, laparotomy (LAP), CLP, and three treatment groups including indomethacin, celecoxib, and aspirin (۲ mg/Kg b.w) taken orally every ۱۲ hours for ۲ days. The rats were sacrificed after ۴۸ hours, and the lung tissue was subjected to determine lipid peroxidation (LP), myeloperoxidase (MPO), glutathione enzyme (GSH)) and inflammatory genes expression (cyclooxygenase-۲ (COX-۲), CD۱۷۷, and MPO). Results: Cecal ligation and puncture caused lung injury by changes in antioxidant enzymes and genes expression (P<۰.۰۵). Treatments with indomethacin, celecoxib, and aspirin as anti-inflammatory compounds significantly improved antioxidant enzymes by reducing LP and MPO levels and genes expression and increasing the level of GSH (P<۰.۰۵). Conclusion: Sepsis caused oxidative damage in the lung tissue, and NSAIDs effectively prevented and improved these injuries.

Keywords:

Sepsis , Inflammation , Antioxidant Activity , Genes Expression , Cecal Ligation , and Puncture Surgery