The effect of hydro-alcoholic extracts of Nigella sativa, Carum carvi, Taraxacum officinale, and royal jelly on the survival of HDF cell line under H۲O۲-induced oxidative stress compared to metformin

Background and aims: Highly active oxygen causes DNA or protein damage. This study aimed to determine the effect of the extract of three medicinal plants, royal jelly, and metformin on the survival of human dermal fibroblast (HDF) cells under oxidative stress induced by H۲O۲. Methods: Hydroalcoholic extracts were prepared from Nigella sativa, Carum carvi seeds, and Taraxacum officinale flower. The growth curve of HDF cells was plotted in Dulbecco’s modified eagle medium (DMEM). The cells were exposed to H۲O۲ stress and treated with different concentrations of the extracts, royal jelly, and metformin. The cells viability was then calorimetrically analyzed during different times in ۶۹-well plates. Results: The exponential growth phase of HDF cells was observed during ۲۴–۷۲-hour growth. High cell survivals were seen during ۷۲ hours of exposure to ۲۵ mg/mL royal jelly (۹۹.۱۳ ± ۳.۲۲%), ۱۵ mg/mL C. carvi extracts and N. sativa (۹۷.۸۷ ± ۳.۲۵% and ۹۶.۲۲ ± ۵.۴۷%, respectively), ۱۰ mg/mL royal jelly (۹۶.۱۴ ± ۴.۷۲%), ۱۶ mg/mL metformin (۹۱.۲۳ ± ۵.۳۴%), and ۸ mg/mL T. officinale (۸۶.۳۱ ± ۷.۴۴%), respectively. These results were significantly higher than the results of ۴۸ and ۲۴ hours of exposure to ۱۵ mg/mL N. sativa (P = ۰.۰۱۳), ۱۵ mg/mL C. carvi (P = ۰.۰۱۹), and ۱۶ mg/mL metformin (P = ۰.۰۲۷). Moreover, royal jelly (۲۵ mg/mL) significantly indicated greater effect on the cell survival compared with the highest concentrations of the extracts and metformin at different exposure times (P = ۰.۰۱۶-۰.۰۳۲). Conclusion: Royal jelly, N. sativa, and T. officinale extracts revealed remarkable effects on the stressed HDF cells; therefore, they can be proposed for treatment of wounds and burns after clinical analysis.

Keywords:

Plant extracts , Royal jelly , Metformin , Oxidative stress , HDF cell line