Evaluation and Diagnosis of Species and Biovars of Brucella among cattles by Multiplex Polymerase Chain Reaction

Background and Aims: Brucellosis is an infectious zoonotic disease responsible for reproductive disorders among animals and significant economic losses to the meat and milk supplychain. The purpose of this study was therefore to evaluate and diagnose the species and strains of Brucella isolates among cattles via multiplex polymerase chain reaction. Materials and Methods: In this study, blood samples were taken from ۲۲ cows diagnosed as having brucellosis by PCR test. Blood samples were cultured in BACTEC vials and incubated at ۳۷ degrees Celsius for ۵ days following which each sample was cultured on Brucella agar and kept for ۳ days under Co۲ condition.The ۱۶SrRNA gene was used as the target for the detection of Brucella genus and the bcsp۳۱ gene was used for the detection of Brucella biovars. The PCR products were electrophoresed on %۱ agarose and compared with standard strains. Results: The PCR results showed a total of ۲۲ positive samples for Brucella among cattles. Out of the ۲۲ cattle positive samples, ۱۹ were identified as B.abortus (biovars ۱, ۲, ۳, ۴, ۵) and ۳ as B. melitensis (biovars ۱, ۲, ۳). Conclusions: Brucella abortus biovars ۱ and ۳ and B .melitensis biovar ۱ are the most prevalent biovars among cattles in Lorestan province, Iran. Moreover, multiplex PCR method could be used to improve the qualitative level and the Brucella diagnosis speed in laboratories.

Keywords:

Brucellosis , PCR , Brucella species , بروسلوزیس , PCR , گونه های بروسلا