Frequency of Plasmid-Mediated Quinolone Resistance Genes among Clinical Isolates of Pseudomonas aeruginosa in Kerman, Iran
Publish place: Iranian Journal of Medical Microbiology، Vol: 11، Issue: 3
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJMM-11-3_002
تاریخ نمایه سازی: 16 مرداد 1402
Abstract:
Background and Aims: plasmid genes are responsible for resistance to quinolones and fluoroquinolones, so the present study aims to identify the presence of qnr and aac genes in clinical isolates of P. aeruginosa.
Materials and Methods: In ۲۰۱۶, ۶۰ strains of P. aeruginosa were isolated from clinical specimens of patients in Kerman hospitals. Antibiotic resistance patterns were evaluated using Kirby-Bauer and microdilution methodsagainst ۱۰ antibiotics according to CLSI criteria Specific primers and polymerase chain reaction were used to detect and amplify qnr and aac(۶)-Ib-cr genes.
Results: Maximum antibiotic resistance was observed against cefexim (۸۰%) and calidixic acid (۷۵%) and minimum resistance against imipenem (۲۵%) and ciprofloxacin (۳۵%). The incidence rate of quinolone resistance genes were as follows: qnr A (۱۶.۶۶%), qnrB (۱۳.۳۳%), qnrS (۱۱/۶۶%) and aac(۶)-Ib-cr (۸/۳۳%).
Conclusions: In the present study, qnrA gene had the highest incidence rates among the studied genes. Because of the importance of antibiotic resistance and the high prevalence of qnr genes found in this study , furthur studies need to be carried out on qnr resistance genes in the regional and national dimension.
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Authors
Sohrab Rajaei
Department of Microbiology, Islamic Azad University, Kerman, Iran
Nadia Kazemi-Pour
Department of Microbiology, Islamic Azad University, Kerman, Iran
Farokh Rokhbakhsh-Zamin
Department of Microbiology, Islamic Azad University, Kerman, Iran
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