Prevalence of Helicobacter pylori vacA allels and cagA gene in the feces of seroposetive children in Kermanshah city

Background and Aim: Helicobacter pylori is the most common cause of Gastritis and indigestion in the human. Considering the importance of this infection and its different prevalence in different regions of Iran, this study was conducted to determine the prevalence of H. pylori vacA allels and cagA gene in the feces of seroposetive children in Kermanshah city as a high-risk province Materials and Methods: In this study, 300 children aged 2 to 9 years were examined and tested for detection of anti IgG. Then DNA extracted from stools of seropositive patients and at the present of specific primers prevalence of cagA gene and vacA alleles including (s1a, s1b, s1c, s2, m1and m2) and were determined. Results: A total of 300 serum samples, 50 cases (16.66%) positive for anti IgG against H. pylori.The findings showed that most strains of H. pylori genotypes vacA s2/m2 are isolated. But the frequency of cagA gene sees in only 10 %. Conclusions: According to the results, the most frequent allele of the gene vacA s2/m2 this area has existing children. To ensure these wider molecular studies in other populations is recommended.

Prevalence of Helicobacter pylori vacA allels and cagA gene in the feces of seroposetive children in Kermanshah city Keywords:

Serum positive children , Stool , Helicobacter pylori , PCR , کودکان , سرم مثبت , مدفوع , هلیکوباکتر پیلوری , PCR