Survey Effect of Peganum harmala, Thymus daenensis, Frulago angulata on wound infection caused by Pseudomonas aeruginosa exotoxin A-producing in laboratory mice

Background and Aim: Burn wound is a susceptible site for incidence of resistant infections. Therefore, research for finding effective drugs against Pseudomonas aeruginosa, the leading pathogen causing burn wound infection, is necessary. The current study aimed at investigating the antibacterial effect of herbs including: Daenensis thyme, Chavill (Frulago angulata), and Harmala on burn infection caused by Pseudomonas aeruginosa and then comparing the obtained results with the results of selected antibiotics on the infection. Methods and Materials: Anti-Pseudomonas activity of plants’ extractions was evaluated by well diffusion method.  The evaluation of burn wound recovery treated with Peganum harmala and daenensis thyme on burn wounds of albinos. Wound area and recovery percentage was measured. The number of neutrophils and lymphocytes, from all infected groups the sample of blood was obtained. Results: The measurement results of the zone of inhibition against Pseudomonas aeruginosa showed better consequences for Thymus daenensis. The number of colonies in the treated group with plants and ciprofloxacin showed significant differences as compared with the control group and the group treated with gentamicin. In counting the white blood cells of infected control group and gentamicin treated group, the number of white blood cells was below the normal level while the number of blood cells in groups treated with medicinal plants and ciprofloxacin were normal. Conclusion: Based on the obtained results of this study on these plant species and their positive activity on treatment of infected burn wounds, it was concluded that these plants can be considered and used as an anti-Pseudomonas.

Keywords:

Thymus daenensis (Thyme) , Peganum harmala( harmala) , Ferulago angulata( chavill) , wound healing , Pseudomonas aeruginosa . , آویشن دنایی , اسفند , چویل , سودوموناس ائروژینوزا , زخم سوختگی