Antibacterial activity of Curcuma longa ethanolic extract against Pseudomonas aeruginosa isolated from burn wound infection and molecular typing of them.

Introduction & Aims: Pseudomonas aeruginosa is important pathogen to produce burn wound infections. Increasing of antibiotic usage for their infections, created antibiotic resistance. Medical herbs have anti-microbial activity. The aims of this study were to determine the antibacterial activity of ethanolic extract of Curcuma amada rhizome against Pseudomonas aeruginosa isolated from burn wound infections and to compare with effects of selected antibiotics and molecular typing of them In-vitro.Methods: First, a sample of ethanolic extract of the plant rhizome was prepared by maceration method. Then its antibacterial activity against ۱۲۲ isolates of Pseudomonas aeruginosa from ۳۰۰ samples of different infection was evaluated by well diffusion and agar serial dilution method. All the resistant isolates were the subjected to PCR assay for bla CTX-M, bla TEM and bla SHV genes that encode ESBL. Results: The results demonstrated that the Curcuma amada rhizome ethanolic extract had been affected against ۹۴ of Pseudomonas aeruginosa isolated (۷۷%). The MIC of the extract for Pseudomonas was۱۴ mg/ml. There was significant difference between the effects of plant and antibiotics on Pseudomonas aeruginosa (P<۰.۰۵). Among ۱۰۰ Pseudomonas aeruginosa isolates that were resistance to Curcuma amada rhizome ethanolic extract, ۵۰ and ۲۰ isolates were CTX-M and TEM producer, respectively. bla – SHV was not detected in any of isolates. Discussion & Conclusions: This study demonstrates that an ethanolic extract of Curcuma amada is effective on Pseudomonas aeruginosa isolated from burn wound infection and its effect is even better than that of selective antibiotics. Further investigations will be necessary.

Keywords:

Pseudomonas aeruginosa , Antibacterial activity , Curcuma longa , Burn wound infection , Molecular typing