Isolation of aroclor۱۲۵۴ degrading bacteria in contaminated soil by transformer oil
Publish place: Iran Agricultural Research، Vol: 35، Issue: 2
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
View: 99
This Paper With 8 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IAR-35-2_004
تاریخ نمایه سازی: 13 شهریور 1402
Abstract:
ABSTRACT- Aerobic biodegradation is an environmental-friendly biological method that allows micro organisms to remove persistent organic pollutants. Aroclor۱۲۵۴ is a mixed compound containing polychlorinated biphenyls (PCBs) along with persistent organic materials. There is no estimate of Aroclor۱۲۵۴ usage and its release into the environment in Iran. A transformer manufacturing plant in Shiraz (located in southwest of Iran) uses transformer oils containing Aroclor۱۲۵۴. Soil samples contaminated by transformer oil were taken from the manufacturing plant’s site and were tested for the presence of certain bacteria, level of degradation and pH. In all samples, ۱۳ bacterial strains were isolated on C-media, and their growth was measured using a spectrophotometer. Of the isolated strains, eight could degrade Aroclor۱۲۵۴. Of these eight strains, three showed weak growth (OD between ۰.۱ to ۰.۱۹۵), three showed low growth (OD between ۰.۲ to ۰.۳) and two showed good growth (OD between ۰.۳ to ۰.۵). Some of the strains grew well during the first week, but their growth decreased and eventually stopped in the second week. For all the growth media on which the bacteria from the soil was separated and cultured, pH decreased as the bacterial growth increased. For some media, in which bacterial growth had stopped, pH increased after ۱۴ or ۲۱ days but it was always below ۷.۰. This indicated that environmental pH or increasing carbon dioxide levels were not suitable for the growth of these strains. However, this trend was not observed in bacteria belonging to Pseudomonas, Curtobacterium and Burkholderia. These bacteria could grow and degrade Aroclor۱۲۵۴ even at pH of ۴.۵. In fact, their degradation efficiency increased at a constant pH of about ۵.۵.
Keywords:
Authors
A. Fararooei
Department of Water Engineering, College of Agriculture, Shiraz University, Shiraz, I. R. Iran
S. Amin
Department of Water Engineering, College of Agriculture, Shiraz University, Shiraz, I. R. Iran
Masoud Noshadi
Department of Water Engineering, College of Agriculture, Shiraz University, Shiraz, I. R. Iran
S. M. Taghavi
Department Plant Protection, College of Agriculture, Shiraz University, Shiraz, I. R. Iran
A. Niazi
Department Institute of Biotechnology, College of Agriculture, Shiraz University, Shiraz, I. R. Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :