Diversity and antibiotic resistance profile analysis of uropathogenic bacteria in human and canines

In Pakistan, Urinary tract infections (UTI) are increasing day by day. The study therefore was designed for isolation identification and antibiotic resistance assessment of UTI causing bacteria. Bacterial pathogens causing UTI in dogs and human are becoming more resistant to antibiotic use. To evaluate the diversity and antibiotic resistance of uropathogenic bacteria, a total of ۸۰ urine samples were collected in sterile containers. A total of ۱۵ urine samples were taken from each gender of human and dogs with UTIs and ۵ from each gender of healthy human and dogs. Samples were cultured for isolation and confirmed by biochemical tests and their antibiotic resistance pattern was checked by Kirby baur disk diffusion test. Samples that were taken from UTI males, E.coli, Proteus spp., Klebsiella spp. and Staph aureus was isolated from ۹۳%, ۶.۶۷%,۲۰% and ۵۳% processed samples whereas from UTI females E.coli, Proteus spp., klebsiella spp., and Staph aureus was isolated ۱۰۰%,۱۳%,۳۳%and ۴۰% of samples respectively. Similarly ۶۰% Staph aureus was isolated from healthy males and females urine samples. While Samples that were taken from UTI dogs E.coli., Proteus spp., Klebsiella spp., and Staph aureus was isolated from ۶۶.۷%, ۶.۶۷%, ۳۳% and ۲۶.۶% of processed samples whereas Samples that were taken from bitches, E.coli., Proteus spp., klebsiella spp., and Staph aureus was isolated from ۷۳%,۱۳%,۲۶.۶% and ۲۶.۶% of samples respectively. In case of healthy dogs and bitches urine smalpes ۶۰% and ۸۰% of Staph aureus was isolated. Antibiotic resistance pattern of isolates E.coli, Proteus spp., klebsiella spp. and Staph aureus from human (male and female) samples showed resistance to ceftriaxone, Levofloxecine, and mild type of resistance to ofloxacin while sensitive to ciprofloxacin and sulfamethazole.The antibiotic resistance pattern of isolates E.coli, Proteus spp., Klebsiella spp. and Staph aureus from dogs and bitches samples showed high resistance to Lincomycin and kanamycin, and lower resistance to norfloxacin and doxycyclin while sensitive to ciprofloxacin, amoxaclin.

Keywords:

E. coli , Klebsiella spp , Proteus Spp , Staph aureus , Urinary tract infections