Effects of dietary exposure of different selenium and iron nanoparticle levels on liver histopathology in beluga (Huso huso) juvenile

Nanotechnology is a key and influential technology in science and industry. With the use of nanotechnology in the aquaculture industry, new and diverse forms of minerals such as iron and selenium are produced on different scales. The need for an optimal level of iron in maintaining fish health is widely established. Selenium nanoparticles have also received attention due to their chemical stability, high bioavailability, biocompatibility and, low toxicity. So, this study aimed to investigate the effect of different levels of selenium nanoparticles (Se-NPs) and iron nanoparticles (Fe-NPs) levels on the histopathology of the liver of the beluga juvenile. ۱۳۵ beluga juvenile (with initial weight of ۲۷۶.۴±۳۲.۳ g and initial length of ۴۰.۰±۲.۰ cm) were randomly distributed in ۲۷ fiberglass tanks at ۹ different treatments with ۳ replicates. Treatments including T۱ (control, no added NPs), T۲ (۱ mg/kg Se-NPs), T۳ (۲ mg/kg Se-NPs), T۴ (۱۰۰ mg/kg Fe-NPs); T۵ (۱۰۰ mg/kg Fe-NPs +۱ mg/kg Se-NPs); T۶ (۱۰۰ mg/kg Fe-NPs +۲ mg/kg Se-NPs); T۷ (۲۰۰ mg/kg Fe-NPs); T۸ (۲۰۰ mg/kg Fe-NPs +۱ mg/kg Se-NPs); T۹ (۲۰۰ mg/kg Fe-NPs +۲ mg/kg Se-NPs). The experimental period lasted ۵۶ days. In order to find the histopathological changes, six fish tissues from each group (two fish of each replicate) were sampled at the end of the experiment. Samples were dehydrated by routine methods and embedded in paraffin wax. They were sectioned by microtome and stained with H & E. Necrosis, vacuolation and fat degeneration were the most tissue lesions observed in the liver.

Keywords:

Nanoparticle , Selenium , Iron , Histopathology , Vacuolation , Necrosis