Cellulase Production by Trichoderma reesei using Sugar Beet Pulp

Cellulase production by the fungus Trichoderma reesei was studied using sugar beet pulp (SBP) as a substrate. The subculture medium was a salt solution consisting of KH۲PO۴, CaCl۲, etc. Fungal cells were sub-cultured in an orbital shaker (۱۸۰ rpm) at ۳۰°C for ۱-۲ generations (two days for each generation) and were then used as an inoculum. Exponential cells were inoculated into a medium containing SBP. Data showed that cellulose in SBP has a potential to be used as a substrate to produce cellulase. The effect of cellulose concentration on cellulase production was investigated. Using the medium composition (the mixture of chemical salts and SBP substrate) and a fermentation time of ۴-۶ days, a maximum cellulase activity of ۰.۴۶ IU/ml of filter paper activity was obtained.

Keywords:

Cellulase , Sugar beet pulp , Trichoderma reesei

Authors

M. Moosavi-Nasab

Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, I. R. Iran

M. Majdi-Nasab

Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, I. R. Iran

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شناسه ملی سند علمی:

JR_IAR-25-1_009

تاریخ نمایه سازی: 19 مهر 1402

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Moosavi-Nasab, M. and Majdi-Nasab, M.,1386,Cellulase Production by Trichoderma reesei using Sugar Beet Pulp,https://civilica.com/doc/1778606

Scientometrics

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Ranking of Shiraz University

Type of center: دانشگاه دولتی

Paper count: 29,589

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