Qualitative Melissopalynology Analysis, Glucose Oxydase Activity, and Antibacterial Effect of Honey Samples from Different Botanical Origin

Background and Aim: This research aims to study the qualitative melissopalynology analysis, Glucose oxydase (GOX) activity, and the antibacterial effect of honey samples from different botanical and geographical origins. Materials and Methods: Five natural honey samples were collected from local beekeepers. The antibacterial effect was carried out towards pathogenic bacteria including Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The antibacterial activity of honey samples was evaluated using six dilutions (۸۰%, ۴۰%, ۲۰%, ۱۰%, ۵%, and ۲.۵%). The bacterial susceptibility to honey was evaluated by the agar well diffusion and broth dilution assays. GOX activity was determined using hydrogen peroxide as a standard with peroxydase and o-dianisidine. Results: The results showed that two honey samples could be classified as monofloral (H۱: Hedysarum coronarium, H۴: Ziziphus sp), whereas honey samples H۲, H۳, and H۵ are multifloral honey. All honey exhibit a good bactericidal effect against pathogenic bacteria. Statistical analysis showed that there was a correlation between GOX level and the antibacterial of honey samples. Conclusion: It can be concluded that honey is an important natural product, which has an antibacterial effect towards pathogenic bacteria. Therefore, honey should be used as an alternative therapy for the treatment of wound infected by multidrug resistant bacteria. Further research should be conducted to evaluate the mechanism and the bioactive compounds in honey.

Keywords:

Antibacterial Activity , Honey , Glucose Oxydase , E.coli , P.aeruginosa , S.aureus. , فعالیت ضد باکتریایی , عسل , گلوکز اکسیداز , اشریشیاکلی , P.aeruginosa , S.aureus.