Efficiency of secondary metabolites produced by Trichoderma spp. in the biological control of Fusarium wilt in chickpea

The present study was aimed to investigate the effects of secondary metabolites produced by five Trichoderma spp. on the control of Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris (FOC) in chickpea. In vitro biocontrol potentialities of Trichoderma spp. against FOC was tested. Trichoderma secondary metabolites were extracted by solvent extraction methods and evaluated against FOC. In vitro tests showed very good inhibitory effects by all Trichoderma spp. against FOC along with an inhibitory rate up to ۷۳.۸% and ۲۷.۸%, for direct and indirect contacts, respectively. Additionally, Trichoderma spp. caused a significant decrease in Fusarium wilt disease severity, in particular, T. polysporum showing ۶۴.۲% of disease severity reduction. The tested secondary metabolites were also effective against FOC with a significant decrease of mycelial growth from ۶% to ۷۶.۹%. Similarly, in vivo tests revealed that secondary metabolites were very active in reducing disease severity. It was found that T. polysporum was the most active with ۵۶.۹% of disease severity reduction. Chickpea resistance is mostly attributed to polyphenolic compounds. The studied Trichoderma spp. and their secondary metabolites could be used as potential and promising antifungal agents in preventing the occurrence of Fusarium wilt in chickpea.

Keywords:

Biological control , Trichoderma , Fusarium oxysporum , secondary metabolites , Chickpea