Pantoea agglomerans ENA۱ as a biocontrol agent of Macrophomia phaseolina and growth enhancer of soybean

On the basis of preliminary in vitro screening tests, a competent strain of Pantoea agglomerans ENA۱ (P. agg. ENA۱) recovered from soybean nodule was evaluated for its antagonistic activity against Macrophomina phaseolina causal agent of charcoal rot of soybean. The results of various in vitro assays showed that P. agg. ENA۱ is capable of exerting strong antagonistic effect against M. phaseolina inhibiting its mycelial growth up to ۸۹% as compared to control. The results showed a significant reduction of the disease as measured in host-plant weight increase, reduced microsclerotial coverage of the host tissues and decreased population of the pathogen in soil. Soils treated with the antagonist in presence of the pathogen resulted in ۴۰% increase in aerial fresh weight and ۶۳% decrease in root and stem surface covered by microsclerotia as compared with control. Furthermore, a significant decrease in the pathogen population ranging from ۷۳ to ۷۶% was observed in sterile and non-sterile soils, respectively. P. agg. ENA۱ is suggested as a potent biocontrol agentthat provides excellent rhizosphere colonization and control of M. phaseolina

Keywords:

Macrophomina phaseolina , Biocontrol , soybean , Pantoea agglomerans , antifungal metabolite