Some properties of α-amylase in the digestive system and head glands of Cryptolaemus montrouzieri (Coleoptera: Coccinellidae)
Publish place: Journal of Crop Protection، Vol: 1، Issue: 2
Publish Year: 1391
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JCP-1-2_002
تاریخ نمایه سازی: 13 آبان 1402
Abstract:
Biochemical characteristics of α-amylase in the digestive system and head glands of Cryptolaemus montrouzieri, a key predator of citrus mealybug, Planococcus citri (Pseudococcidae), were studied. The major isoform of α-amylase with the same molecular weight was detected in both gut and head glands loaded on polyacrylamide-starch gel electrophoresis. Moreover, a minor band with much lower intensity was observed in zymogram analysis of gut. Results showed that the specific activity of α-amylase from head glands (۰.۸۹ ± ۰.۰۲ μmol/min/mg protein) was significantly more than that of digestive system (۰.۷۶ ± ۰.۰۱ μmol/min/mg protein) in common condition (temperature equal to ۲۵ ± ۱ oC). The optimal pH and temperature for α-amylases activity were determined to be nearly ۴ and ۵۰°C in digestive system and ۶ and ۶۰ °C in head glands, respectively. EDTA (Ethylenediamine tetra acetic acid), Mg۲+, Na+, Co۲+, Fe۲+ and Ca۲+ inhibited the enzyme activity but Ba۲+, Zn۲+, Hg+ and K+ enhanced enzyme activity in digestive system. EDTA and all tested metal ions except Ba۲+ inhibited the enzyme activity of head glands. Detectable levels of α-amylase activity in the insect reflect adaptation of the coccinellid for using starch granules or sugars (honeydew; sugary excreta of homopterans; and nectar) as a source of food in addition to predatory habits.
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Authors
Fatemeh Ahmadi
Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran.
Abbas Khani
Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran. Tel. and Fax: +۹۸-۵۴۲-۲۲۴۲۵۰۱; E-mail address: abbkhani@yahoo.com
Mohammad Ghadamyari
Department of Plant Protection, Faculty of Agricultural Science, University of Guilan, Rasht, Iran.