Molecular identification of formae specialis and racial identity in Iranian strains of Fusarium oxysporum f. sp. lycopersici: detection of avirulence genes
Publish place: Journal of Crop Protection، Vol: 6، Issue: 1
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JCP-6-1_007
تاریخ نمایه سازی: 13 آبان 1402
Abstract:
Fusarium oxysporum f. sp. lycopersici (Fol) is the causal agent of vascular wilt in tomato, an important plant disease in Iran. Four monogenic resistance genes in tomato are used for identification of races of Fol and their corresponding avirulence genes Avr۱, Avr۲ and Avr۳ were identified in pathogen one of which, Avr۲, is f.sp. specific. Hence they can serve as reliable markers for racial identity and f.sp discrimination. These markers have been used for strains from other countries except Iran. Furthermore, a point mutation in Avr۳ can lead to enhanced virulence of Fol on a susceptible tomato cultivar. To identify forma specialis and racial identity, Avr genes were studied in a collection of Iranian strains. Results revealed that PCR assay is very efficient in distinguishing between non-pathogenic and low virulence strains and in the vast majority of strains, avirulence genotype was consistent with Fol race۱. Furthermore, to determine whether allelic variation of Avr۳ could separate strains of different degrees of virulence, Avr۳ wassequenced in Fol strains with high and low virulence. The resultsrevealed that allelicvariation of Avr۳ was not correlated with degree of virulence in Iranian strains.
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Authors
Elahe Rabiei-Motlagh
Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Hamid Rouhani
Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Farhad Shokouhifar
Plant Science Research Institute, Ferdowsi University of Mashhad, Mashhad, Iran.
Mahrokh Falahati Rastegar
Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Parissa Taher
Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
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