Effect of Aerobic Training and L-carnitine on MDA, GPX and Hippocampal Neurogenesis in Diabetic Rats

Objective: L-carnitine is associated with an increase in antioxidant enzymes activities and reduction in oxidative damage. The aim of this study was to evaluate the effect of aerobic training and L-carnitine cconsumption on hippocampal oxidative stress and neurogenesis factors in diabetic rats. Materials and Methods: In this experimental study, ۴۵ male wistar rats were divided into six groups including sham (۵ rats), healthy control (۸ rats), diabetic control (۸ rats), diabetic receiving L-carnitine (۸ rats), diabetic receiving aerobic exercise (۸ rats), diabetic receiving L-carnitine and aerobic exercise (۸ rats). Rats with serum glucose level higher than ۳۰۰ mg/dL were considered diabetic. L-carnitine supplemented dose was ۱۰۰ mg per day. The aerobic exercise protocol including five sessions per week started at a speed of ۱۰ for ۲۰ min at a zero-degree grade in the first week and gradually reached a speed of ۲۰ for ۴۰ min at a grade of ۵ in the sixth week. The Hippocampal tissues of the dependent variables (BDNF, MDA and GPX) were measured by ELISA ۲۴ hours after the last session of the exercise program. One-way ANOVA and Tukey's post hoc test at P-value< ۰.۰۵ were used to analyze the data. Results: The results of the study revealed that aerobic exercise and L-carnitine consumption have a significant effect on BDNF (P-value= ۰۰۱), MDA (P-value= ۰۰۱) and GPX (P-value= ۰۰۱) of the diabetic rats. Conclusion: According to the results, it is suggested that diabetic patients use aerobic exercise and supplementation with carnitine with caution and physician advice.

Keywords:

Aerobic exercise , L-carnitine , Diabetes , BDNF , MDA , GPX