Chitinolytic Activity of Native Pseudomonas fluorescens Strains

The chitinase producing ability of Pseudomonas fluorescens strains viz., PF۱, PB۲ and FP۷ was evaluated in a culture medium with and without a chitin source. The addition of ۱% (v/v) chitin in culture medium significantly increased the bacterial population and chitinase activity. Among three strains tested, FP۷ responded well to the addition of chitin by producing ۳۱.۲% increased chitinase in culture. Western blot analysis with chitinase antibody detected six and five chitinase isoforms in culture inoculated with FP۷ and PF۱, respectively.

Keywords:

Biological control , Chitin , Chitinase , Pseudomonas fluorescens