A Functional Non-Dairy Beverage Produced from Jujube Extract Using Probiotic Lactic Acid Bacteria

In this research, production of a probiotic drink based on jujube extract by means of fermentation with Lactobacillus plantarum and Lactobacillus delbrueckii as probiotic lactic acid bacteria was studied. The fermentation was performed for ۷۲ hours at ۳۷°C. The changes in microbial population, pH and titratable acidity as well as sugar and organic acid metabolism during the fermentation period were evaluated. In addition, before and after fermentation, the changes in total phenolic compounds and antioxidant activity in the extract were also investigated. Eventually, different drink formulations were developed employing fermented and non-fermented jujube extract, carbonatation, and sour cherry concentrate. Then, sensory properties of the formulated drinks were evaluated. Appropriate growth of L. plantarum and L.delbruckii resulted in an increase in acidity to ۱.۸۶ and ۱.۷۵, and a decline in pH to ۳.۴ and ۳.۵۶, respectively, after ۷۲ hours. Glucose and fructose were significantly consumed by the strains. Citric acid concentration dropped to ۱.۱۲ and ۵.۸ g L-۱ in the extract fermented by L. plantarum and L. delbrueckii, respectively, after ۷۲ hours. At the end of fermentation, ۲۳.۸ and ۱۱.۴ g L-۱ lactic acid was produced by L. plantarum and L. Delbrueckii, respectively. The contents of phenolic compounds and antioxidant activity in jujube extract after fermentation were significantly increased.The results of sensory evaluation of different drink formulations showed that the carbonated drink containing jujube extract fermented by L.plantarum mixed with sour cherry concentrate obtained the highest score among different drink formulations.

Keywords:

Antioxidant activity , Fermentation , Lactobacillus , Ziziphus jujube.