Expression of pluripotent stem cell markers in mouse uterine tissue during estrous cycle
Publish place: Veterinary Research Forum، Vol: 7، Issue: 3
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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JR_VRFAN-7-3_001
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
It was assumed that uterine stem cells are responsible for the unique regenerative capacity of uterine. Therefore, the aim of the present study was to investigate the expression of the pluripotent stem cell markers in the mice uterine tissue during different stages of estrous cycles. Twelve virgin female NMRI mice (۶ to ۸ weeks old) were considered at proestrus, estrus, metestrus and diestrus according to the cell types observed in the vaginal smear and underwent hysterectomy operation. Quantitative real-time polymerase chain reaction (PCR) and immunohistochemical staining for pluripotent stem cell markers (SOX۲, OCT۴, KLF۴, and NANOG) were performed. Immunofluorescence staining revealed that expression and localization of the pluripotency markers SOX۲, OCT۴, KLF۴, and NANOG at the protein level were not different throughout estrous cycle. Also, mRNA of pluripotency markers was detected in all tested samples. However, there were no significant differences in their genes expression at each stage and during the estrous cycle. Different hormonal profile during the estrous cycle could not affect expression of pluripotent stem cell markers in uterine tissue.
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Authors
Kolsum Choobineh
Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran
Saeed Zavareh
Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran
Mojdeh Salehnia
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Mohamad Taghi Ghorbanian
Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran
Seyed Hassan paylakhi
Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran
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