Ventrolateral periaqueductal gray exogenous and endogenous histamine attenuates sciatic nerve chronic constriction injury-induced neuropathic pain through opioid receptors
Publish place: Veterinary Research Forum، Vol: 12، Issue: 4
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_VRFAN-12-4_006
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
The aim of the present study was to investigate the effects of intra-ventrolateral periaqueductal gray (vlPAG) microinjection of histamine and thioperamide (a histamine H۳ receptor antagonist/inverse agonist) on neuropathic pain. To explore the possible mechanism, naloxone was microinjected alone or in combination with histamine and thioperamide. Neuropathic pain was induced by the left sciatic nerve chronic constriction injury. Both the right and left sides of vlPAG of the brain were surgically cannulated. Cold allodynia and mechanical hyperalgesia were recorded by acetone evaporation and von Frey filament tests. Areas under curve of allodynia and hyperalgesia were calculated. Histamine (۰.۵۰ and ۲.۰۰ µg per site), thioperamide (۴.۰۰ µg per site) and thioperamide (۴.۰۰ µg per site) before histamine (۲.۰۰ µg per site) suppressed cold allodynia and mechanical hyperalgesia after microinjection into the vlPAG. Microinjection of naloxone (۰.۲۵ and ۱.۰۰ µg per site) into the vlPAG had no effect on cold allodynia and mechanical hyperalgesia. The anti-allodynic and anti-hyperalgesic effects induced by microinjection of histamine (۲.۰۰ µg per site) and thioperamide (۴.۰۰ µg per site) into the vlPAG were inhibited by prior microinjection of naloxone (۱.۰۰ µg per site) into the same site. The above-mentioned agents did not alter locomotor activity. Based on our present results, it was concluded that exogenous (by histamine microinjection) and endogenous (by thioperamide microinjection) histamine of the vlPAG might contribute to the descending pain control mechanisms through a naloxone-sensitive mechanism.
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Authors
Sara Salimi
PhD Candidate, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Esmaeal Tamaddonfard
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Farhad Soltanalinejad-Taghiabad
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
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