Intra-vitreal injection of methotrexate in experimental endotoxin-induced uveitis in rabbit

Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_VRFAN-9-4_004

تاریخ نمایه سازی: 25 آبان 1402

Abstract:

Uveitis is a major cause of vision loss. Methotrexate (MTX) has been widely used in uveitis due to its relatively safe profile. The purpose of this study was to evaluate the effects of two different dosages of MTX via intra-vitreal administration for treatment of endotoxin induced uveitis (EIU) in an experimental model. Thirty-five healthy rabbits were randomly divided into four groups and all animals were tolerated intra-vitreal injections. The first group received normal saline (NS), the second group received normal saline plus Salmonella typhimurium lipopolysaccharide endotoxin (LPS), (NS+LPS), the third group received ۴۰۰ μg MTX plus LPS (LPS+MTX ۴۰۰) and the fourth group received ۸۰۰ μg MTX plus LPS (LPS+MTX ۸۰۰). Intra-ocular inflammation was evaluated by clinical examination scoring during ۷ post-injection days and histopathological examination at the end of study. Kruskal-Wallis and Mann-Whitney U tests were used to compare the histopathological and clinical scores. According to the clinical examinations, all groups demonstrated higher uveitis score than group ۱ on first post-injection day. Also, groups ۲ and ۳ showed greater uveitis score than group ۴. On the third, fifth and seventh post-injection days, clinical uveitis score in groups ۲, ۳ and ۴ was significantly higher than group ۱. The mean histopathological inflammation intensity scores in groups ۲, ۳ and ۴ were significantly higher than group ۱. Single intra-vitreal injection of ۴۰۰ μg and ۸۰۰ μg of MTX did not show significant anti-inflammatory effects on EIU in rabbits.

Authors

Mohammad Abbaszadeh Hasiri

Department of Clinical Science, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

Effat Baghaei Moghaddam

Department of Clinical Science, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

Mohammad Reza Khalili

Poostchi Ophthalmology Research Center, Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran

Amin Hossein Amini

Department of Clinical Science, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

Masoomeh Eghtedari

Poostchi Ophthalmology Research Center, Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran

Mohammad Azizzadeh

Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Hooman Razmi

Poostchi Ophthalmology Research Center, Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran

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