Anti-quorum sensing effects of Licochalcone A and Epigallocatechin-۳-gallate against Salmonella Typhimurium from poultry sources
Publish place: Veterinary Research Forum، Vol: 11، Issue: 3
Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
View: 66
This Paper With 7 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_VRFAN-11-3_011
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
Quorum sensing (QS) is a cell density-dependent mechanism used by many pathogenic bacteria for regulating virulence gene expression. Inhibition or interruption of QS by medicinal plant remedies has been suggested as a new strategy for fighting against antibiotic-resistant bacteria. This study aimed to assess the impact of sub-inhibitory concentrations of licochalcone A (LAA) and epigallocatechin-۳-gallate (EGCG) as natural plant products on the QS-associated genes(sdiA and luxS)expression. The PCR test was used to confirm the presence of sdiA and luxS genes in ۲۳ S. Typhimurium isolates from poultry. The quantitative real-time PCR assay was used to analyze the expression of sdiA and luxS in S. Typhimurium isolates in response to the treatment with sub-inhibitory concentrations of LAA and EGCG at ۴۵-min time point. All S. Typhimurium isolates showed the presence of sdiA and luxS genes (۱۰۰%). As result, the expression of QS-related genes was significantly reduced in S. Typhimurium isolates following treatment with LAA and EGCG. In conclusion, LAA and EGCG showed anti-QS activity with down-regulation of both sdiA and luxS genes in S. Typhimurium, suggesting potential therapeutic use of them against salmonellosis. However, it must be pointed out that the safety and efficiency of these compounds need more thorough research.
Keywords:
Authors
Somayyeh Hosseinzadeh
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Habib Dastmalchi Saei
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Malahat Ahmadi
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Taghi Salehi
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :