Antioxidant property of Plantago major leaf extracts reduces testicular torsion/detorsion-induced ischemia/reperfusion injury in rats
Publish place: Veterinary Research Forum، Vol: 11، Issue: 1
Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_VRFAN-11-1_005
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
The present study was aimed to determine the protective effects of Plantago major L (PM) leaf extracts on the testicular torsion/detorsion (T/D)-induced ischemia/reperfusion (I/R) injury in rats. Twenty-four mature male Sprague-Dawley rats, weighing ۲۰۰-۲۲۰ g, were selected. They were randomly divided into four groups of six animals each: Sham (sham-operated rats; all the surgical steps were performed but T/D was not induced), TDC (control group; T/D was induced and the right testicular torsion of ۷۲۰° lasting two hours was followed by detorsion), TDP۵۰ (T/D-operated rats received ۵۰.۰۰ mg kg-۱ of PM extract daily for seven days intraperitoneally after detorsion) and TDP۱۰۰ (T/D-operated rats received ۱۰۰ mg kg-۱ of PM extract daily for seven days intraperitoneally after detorsion). After seven days of treatment, the right testicles were collected. Histopathological and biochemical analyses including levels of malondialdehyde (MDA) and catalase (CAT) and peroxidase activities were determined in testicular tissues of the rats. Tissue sections were taken from testis, Hematoxylin-Eosin staining was done, and the slides were examined by a light microscope. The level of MDA was significantly increased in the testes of the TDC group. The CAT activity levels were decreased significantly after I/R. The post-torsion treatment with PM, particularly at ۱۰۰ mg kg-۱, prevented the increase in lipid peroxidation and reduced the CAT activity levels. The PM also prevented I/R-induced cellular damage and histological changes in the testicular tissues. According to the results of the current study, PM leaf extracts had significant positive effects on the testicular T/D-induced I/R injury. The possible mechanism of reduction in biochemical and histological injuries by PM extracts could be due to antioxidant property.
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Authors
Masoumeh Moradi-Ozarlou
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Sara Javanmardi
Department of Clinical Sciences, Faculty of Veterinary medicine, University of Tabriz, Tabriz, Iran
Hossein Tayefi Nasrabadi
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
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