Attenuating effects of Mangifera indica leaves ethanolic extract against acetamiprid induced reproductive toxicity in male guinea pigs

Acetamiprid (ACP) belonging to the neonicotinoid family used against wide array of pests in agriculture and domestic purposes. In this study, we evaluated the attenuating effects of ethanolic extract of Mangifera indica leaves (EEMI) in averting reproductive toxicity caused by ACP in male guinea pigs. Thirty male guinea pigs were randomly assigned to five treatment groups (n = ۶). Group ۱ (T۰) received distilled water orally; group ۲ (T۰-) was given ۸۰ mg kg-۱ of ACP and groups ۳, ۴ and ۵ were treated, respectively, with EEMI at doses of ۵۰, ۱۰۰ and ۲۰۰ mg kg-۱ plus ACP. After ۹۰ days, the reaction time, sexual organ weights, sperm count, motility and anomalies, spermatozoa with entire plasma membrane, testicular histology, serum testosterone concentration, testicular malondialdehyde (MDA) level, reduced glutathione (GSH) concentration, testicular superoxide dismutase (SOD) and catalase (CAT) activities were assessed. Co-administration of EEMI significantly reduced the reaction time, sperm anomalies and testicular MDA, SOD and CAT levels compared to the T۰- group. Co-treatment of EEMI significantly alleviated sperm count and motility, percentage of spermatozoa with the normal plasma membrane, serum testosterone concentration, accessory sex gland weights, and testicular GSH concentrations. The ACP treatment induced cell membrane degradation in the testis and this effect was prevented with the addition of EEMI. In conclusion, ACP negatively affected the animal reproductive function and induced oxidative stress. The addition of EEMI alleviated the toxic effects of ACP on the reproductive function of male guinea pigs.

Keywords:

acetamiprid , Guinea pigs , Mangifera indica , Oxidative stress , Reproductive functions