Evaluation of TRAF۳IP۲ Gene Expression in Brain Tumor Tissue of Patients with Glioblastoma Multiforme in Comparison to Non-Tumoral Brain Tissue

Background: Glioblastoma, not otherwise specified (NOS), is the most common primary malignant brain tumor. The TRAF۳IP۲ gene is an upstream regulator responsible for activating multiple proinflammatory pathways that could influence tumor size, angiogenesis, aggressiveness, and metastasis. In the present study, we aimed to investigate and assess the TRAF۳IP۲ gene expression in brain tumor tissue of patients with glioblastoma, NOS and compare it with non-neoplastic brain tissue.Method: In this case-control study, biopsies were obtained from ۱۵ surgically glioblastoma, NOS removed block samples and ۱۵ non-neoplastic brain tissue samples containing normal white and gray matter as controls. Ribonucleic acid (RNA) was isolated and reverse-transcribed to complementary DNA (cDNA). Quantitative polymerase chain reaction (qPCR) was then carried out to measure TRAF۳IP۲ gene expression.Results: We evaluated data from ۳۰ cases, divided into two groups: case (N = ۱۵) and control (N = ۱۵). Based on our data, the expression of the TRAF۳IP۲ gene was ۶.۹۵ ± ۰.۶۵ times higher in glioblastoma multiforme tissue compared with controls (P < ۰.۰۵). We also found no significant difference in TRAF۳IP۲ gene expression between genders (P = ۰.۴۵۲), and there was no significant correlation between TRAF۳IP۲ gene expression and age (P = ۰.۷۴۵).Conclusion: The expression of the TRAF۳IP۲ gene was almost seven times higher in glioblastoma, NOS brain tissue compared with normal brain samples. This finding could have significant clinical and therapeutic implications.

Keywords:

Glioblastoma multiforme , Gene expression , Case-Control Study