Evaluation of the Cytotoxic Effects of Ciprofloxacin on Human Glioblastoma A-۱۷۲ Cell Line

Background: Glioblastoma multiforme, the most common, aggressive malignant brain tumor which affects patients of all ages, is principally resistant to treatment. Ciprofloxacin is an antibiotic that belongs to the fluoroquinolones. There are welldocumented observations which indicate that ciprofloxacin has substantial anti-proliferative, apoptotic, cytotoxic and oxidative stress activities on various tumor cell lines.Methods: We exposed the glioblastoma A-۱۷۲ cell line to ciprofloxacin for ۲۴, ۴۸ and ۷۲ h. Cytotoxicity was measured using MTT assay. The levels of Bax as an apoptotic and Bcl-۲ as an anti-apoptotic protein were measured by ELISA and oxidative stress by the malondialdehyde assay.Results: Ciprofloxacin induced tumor cell death in a dose-dependent manner with an IC۵۰ value of ۲۵۹.۳ μM at ۷۲ h. We observed an increase in Bax levels, a decrease in Bcl-۲ concentrations and increased Bax/Bcl-۲ ratio under the influence of ciprofloxacin. Malondialdehyde levels, as an important marker of oxidative stress, increased in the human glioblastoma A-۱۷۲ cell line.Conclusion: These results indicated that ciprofloxacin had anti-tumor, cytotoxic and apoptotic effects in the human glioblastoma A-۱۷۲ cell line which might be useful as an adjuvant added to a glioblastoma multiforme chemotherapeutic protocol in the future.