Screening Bread Wheat Genotypes for High Molecular Weight Glutenin Subunits and Some Quality Parameters

High Molecular Weight Glutenin Subunits (HMW-GS) compositions of ۱۲۲ genotypes from bread wheat crossing block were investigated in terms of some quality traits such as grain Protein Content (PC), Sodium Dodecyl Sulphate (SDS), the Particle Size Index (PSI), and Thousand Kernel Weight (TKW), by using SDS-PAGE. In total, ۱۲ different HMW-GS combinations were determined. Considerable diversity in terms of three Glu-A۱, Glu-B۱ and Glu-D۱ loci were identified. In Glu-A۱ locus, ۱/۲*, ۱ and ۲* alleles were found with the frequency of ۲.۵, ۱۲.۳ and ۸۵.۵%, respectively. Whereas, in Glu-B۱, out of ۷ reported alleles, ۷+۸ (۲۰.۵%) and ۱۷+۱۸ (۱۷.۲%) were detected. Existence of ۲ alleles at the locus Glu-D۱ was revealed; in fact, ۵۴.۱% of them demonstrated the subunits ۵+۱۰ correlated with good bread making properties. The Glu-۱ score of genotypes ranged from ۶ to ۱۰. Among the genotypes, only ۲۳ (۱۸.۹%) had ۱۰ Glu-۱ quality score value. In the evaluation using the Genotype-Traits (GT) Biplotgraph, PC and PSI were involved in section I while SDS sedimentation value and Glu-۱ score were involved in section II. On the other hand, section III included the only TKWwhich was negatively associated with other traits. The desired genotypes can be used for the crossing programs to improve technological quality of bread wheat.

Keywords:

Biplot , HMW-GS , Landraces , Quality