Protein Patterns and Larvicide Activity of Crystalline Inclusions of Bacillus thuringiensis ssp. kumamotoensis DSM ۶۰۷۰

Morphological and electrophoretic analysis of the crystalline inclusions (parasporal crystals) of sporulated cultures of B. thuringiensis ssp. kumamotoensis DSM ۶۰۷۰ (Bt ۶۰۷۰) was conducted via phase contrast and scanning electron microscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The activity of the spore-crystal suspensions against house fly (Musca domestica) larvae was also assessed. Bipyramidal and smaller, irregular shaped crystals were observed in the sporulated cultures. ۱۳۰, ۷۵ and ۲۵ kDa bands were detected in the protein pattern. The presence of ۲۵ kda proteins in Bt۶۰۷۰ has not been reported earlier. The spore-crystal suspension showed significant larvicide activity against housefly larvae. Larvicide activity of B. thuringiensis ssp. kumamotoensis against any dipteran species has not been detected yet. Further studies are needed on identifying the dipteran- active fraction.

Keywords:

Bacillus thuringiensis , Parasporal crystals , Polyacrylamide gel-electrophoresis , Scanning electron microscopy