Chlorophyll Content, Chloroplast Ultrastructure and Transcriptome Analysis in Wild-type and Yellow-bud-mutant Hot Peppers

The yellow-bud mutant hot pepper, ۹۶-۱۴۰YBM, which exhibits a yellow leaf phenotype in its young leaves but whose matured leaves are green, was isolated from wild type ۹۶-۱۴۰ in this study. The results of photosynthetic pigment determination and chloroplast ultrastructure observation revealed that the young mutant leaves displayed Chl a+b and Cars content, increased Chl a/b and Car/Chl a+b ratios, and delayed chloroplast development compared with the wild type leaves. Here, we obtained ۹۵,۷۱۴ transcripts from cultured yellow-bud mutant yellow leaves and cultured wild-type seedling leaves using the Illumina HiSeq-۲۰۰۰ (Illumina Inc., USA) platform. A total of ۴۲,۳۸۴ unigenes were identified, among which ۳۷,۹۴۹ were annotated using gene descriptions or gene ontology terms. Based on Differentially Expressed Genes (DEG) analysis, ۱,۰۵۶ of the ۱,۱۰۱ DEGs were annotated in the Nr database, and ۳۰۲ unigenes were mapped to ۱۳۰ pathways using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database. Finally, we found that ۶ pathways were related to chloroplast and chlorophyll biogenesis.

Keywords:

Hot pepper , Chloroplast , Transcriptome , Yellow-bud mutant , Wild type