In vitro assay of targeted drug delivery platform based on ZIF-۶۷nanocarrier containing curcumin on MCF-۷ cells

The targeted delivery of anti-cancer drugs exclusively to tumor cells presents an appealingstrategy as it amplifies the therapeutic effectiveness compared to non-targeted medications [۱]. Toenhance the cellular uptake of chemotherapeutic agents, nanoscale delivery systems incorporatingpolyethylene glycol (PEG) as a surface covering, along with aptamer-conjugated nanoparticlescapable of specifically binding to proteins found on the surface of tumor cells, have emerged aspromising solutions. In this study, the Epithelial cell adhesion molecule (EpCAM), which iscommonly overexpressed in solid cancers and has recently been identified as a marker for cancerstem cells, was utilized. Ultimately, The ZIF-۶۷ pnanoparticles encapsulating curcumin(Ccm@ZIF-۶۷@PEG@EpCam NPs) were fabricated in-vitro environment and characterized byXRD, SEM, TEM, DLS, TGA and BET methods [۲]. Then its release was investigated at two pHof ۵.۴ citrate buffer and ۷.۴ saline phosphate buffer. Finally, the cytotoxicity of the syntheticnanoplatform was evaluated by MTT assay on human breast cancer cell line (MCF-۷) [۳].

Keywords:

polyethylene glycol , aptamer , nanoparticle , Epithelial cell adhesion molecule , Zeoliticimidazolate framework-۶۷