Phytochemical Analysis, Antioxidant Activity and Ecological Requirements of Capparis spinosa L. in Golestan and Semnan Provinces (North of Iran)

Natural antioxidants have an important role against damage by ROS. This research had been carried out about ecological characters, phytochemical and antioxidant activity of Capparis spinosa L. in Semnan and Golestan province. So in much field observation, different parts of C.spinosa were collected from two natural habitats in Golestan (۲۰۰ m) and Semnan province (۲۱۰۰ m) during August to September ۲۰۱۱. Methanolic extracts were obtained by maceration, TP (total phenol) and TF (Total flavonoid) were determined by spectro photometrically method and the antioxidant capacity were obtained by TAC, RP and DPPH methods. Results showed that Capparis spinosa L. (Kabar) is an edible plant which was growing wild in clay loam to sandy clay loam soils. In both regions TF and TP content in buds and flowers extracts were highest, especially in ۲۱۰۰ m.  Antioxidant activity of buds and flower extracts (IC۵۰) were the highest (۱.۲۷±۰.۱, ۴.۶۶±۰.۴۲ μg/ml) in ۲۱۰۰ m, especially in DPPH method and the lowest ‎ content belongs to fruit extract in ۲۰۰m. These data will be confirmed the traditional uses of C. spinosa as an antioxidant and anti-inflammatory to treat of many current ailments.

Keywords:

antioxidant capacity , Capparis spinosa L , Ecological requirements , Golestan and Semnan Provinces , Phytochemical (TF , TP)