Evaluation of Camelina sativa Doubled Haploid Lines for the Response to Water-deficit Stress

Camelina is low input and re-emergent medicinal and oilseed crop that it needs a lot of research to make the most of its genetic potential. This study aimed to evaluate the water deficit tolerance of six genotypes (Soheil cultivar and DH083, DH095, DH111, DH116, and DH133 doubled haploid lines)of camelina for some traits in the greenhouse and laboratory conditions. In the greenhouse condition, the response of camelina genotypes to irrigating regime (once every 4, 7 and 10 days) was investigated for some agro-morphological traits. This experiment was done in a randomized complete block design. Analysis of variance showed that the studied genotypes, as well as drought stress levels, were significantly different in terms of plant height, root length, root weight, grain yield, biological yield and harvest index. Mean comparison results also showed that the Soheil cultivar had the highest grain yield (3.03 g/m2). At the laboratory section and the cotyledon culture and callus induction experiment, six lines were evaluated by four levels of PEG 6000 (0, 10, 20 and 30%) for water-deficit stress in a randomized completely design. The callus growth rate, relative callus growth rate and relative callus water content under stress conditions were also studied. There was a significant difference between studied genotypes in studied traits under stress conditions and between different stress levels. In this section, the Soheil cultivar was identified as high tolerant cultivar among other genotypes due to its relative water content (8.3%) and higher callus growth rate (4.2%). So the cultivar Soheil is considered as the least susceptible to dehydration in terms of greenhouse studies and callus induction.

Evaluation of Camelina sativa Doubled Haploid Lines for the Response to Water-deficit Stress Keywords:

Water deficit , Callus , Camelina sativa L , Cotyledon