Oral Candida colonization and anti-fungal susceptibility pattern in patients with hematological malignancy

Background and Purpose: Candidiasis is considered as one of the most important fungal infections in hematological malignancy. Candidiasis is considered as the most important cause of disease and mortality in hematological malignancy and antifungal prophylaxis is important (remove highlighted text).Objectives: The aim of study is to identify and evaluated antifungal susceptibility pattern in patients with hematological malignancyMaterial and Methods: In this study, samples were collected from the oral cavity of ۱۰۰ patients and Candida colonization was confirmed by fungal culture. Isolated Candida strains were identified by ITS-PCR. In vitro antifungal susceptibility tests against fluconazole (replace “fluconazole” with “azoles”), amphotericinB and caspofungin were performed according to CLSI M۲۷-A۳/S۴. Results: Demographics, comorbidities, distribution of Candida species, antifungal susceptibility were analyzed. Study participants included ۱۰۰ patients. The mean age of patients was ۱۵.۴۸% ±۴۸.۷۴ years were in the range of ۱۷ to ۸۴ years and in terms of sex distribution, ۶۴% were male and ۳۶% were female. In terms of the distribution of underlying hematologic malignancy, ۲۷% had lymphoma. The most commonly isolated species among patients were ۴۹% C. albicans, ۳۱% C. glabrata, ۱۰% Co-colonization of C. albicans and C. with C. glabrata .The overall resistance of C.albicance was %۵ to fluconazole%۲ to amphotericinB.C. glabrata showed %۱۱ resistance to fluconazole and was susceptible to amphotericinB.All candida spp.isolated from patients were susceptible to caspofungin.Conclusions: The high rate of colonization of Candida species, especially the significant increase in the frequency of Candida glabrata in patients with blood malignancies and the gradual increase in resistance to fluconazole, necessitate a change in the use of antifungal drugs for the prevention and experimental treatment of hematological malignancy.

Keywords:

Candida , C. albicans , C. Glabrata , Hematologic malignancy