Effects of Bunium persicum essential oil on the reduction of spore germination, growth, and expression of FUM۱ and FUM۱۴ genes in Fusarium verticillioides isolates
Publish place: Current Medical Mycology، Vol: 7، Issue: 2
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
View: 122
This Paper With 8 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_CUMM-7-2_003
تاریخ نمایه سازی: 11 آذر 1402
Abstract:
Background and Purpose: Black Cumin of Kerman (Bunium persicum) is an Iranian plant that is commonly used as an antispasmodic, carminative, and antimicrobial substance. The present study aimed to assess different components of the essence of B.persicum and its effect on antifungal activity, spore germination inhibition, and expressions of FUM۱ and FUM۱۴ genes in Fusarium verticillioides strains.Materials and Methods: The essence was extracted by hydrodistillation and analyzed through gas chromatography-mass spectroscopy. A broth microdilution method was used for the determination of the minimum inhibitory concentration (MIC). In addition, the expression of FUM۱ and FUM۱۴ genes of toxigenic F. verticillioides was assessed byusing the real-time polymerase chain reaction (RT-PCR) technique.Results: Based on the findings, most of the essence consisted of γ-terpinene (۱۵.۵۶%),propanal, and ۲-methyl-۳-phenyl (۱۴.۱۸%). The oil showed a good antifungal activity(mean MIC value: ۲۵۵۶.۸ μg/ml) as well as the inhibition of spore germination and mycelial growth (P<۰.۰۵). The RT-PCR demonstrated that the expression levels of FUM۱ and FUM۱۴ of B. persicum-treated F. verticillioides were ۰.۴۳ and ۰.۵۳ folds ower than the control samples, respectively.Conclusion: These findings revealed that the essential oil of B. persicum has different components responsible for the inhibition of mycelial growth and spore germination of F. verticillioides as well as reduction of expressions of FUM۱ and FUM۱۴ genes involving fumonisin production.
Keywords:
Antifungal activity , Bunium Persicum , FUM۱ and FUM۱۴ genes , Fusarium verticillioides , real time-PCR
Authors
Asad Balal
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranUniversity of Tehran
Aghil Sharifzadeh
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Hojjatollah Shokri
Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
Alireza Khosravi
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran