Evaluation of miR-۱۴۶a expression level in macrophages exposed to Candida glabrata

Background and Purpose: MicroRNAs are small non-coding RNAs with ۱۹۲۴- nucleotides in length. Up- or downregulation of many miRNAs has been shown by stimulation of Toll-like receptors (TLRs) in the innate immune system. Up-regulation of miR-۱۴۶a has been reported by both TLR and heat-killed Candida albicans. In this study, we aimed to evaluate the expression of miR-۱۴۶a in cultured monocyte-derived macrophages (MDMs) infected by Candida glabrata at ۱۲, ۲۴, and ۴۸ hours. Materials and Methods: miR-۱۴۶a expression was evaluated by qRT-real time polymerase chain reaction (PCR) at three time points in C. glabrata-infected MDMs. The data was analyzed using repeated measures ANOVA. Results: miR-۱۴۶a expression was down-regulated in infected MDMs compared to the control group (p <۰.۰۱۸). The expression of miR-۱۴۶a was at its highest level at ۴۸ h, as compared to ۱۲ and ۲۴ h (p <۰.۰۱۸). The differences between the experimental group compared to the control group were statistically significant (p <۰.۰۱۸). Conclusion: These results suggest that miR-۱۴۶a can be involved in regulating macrophage function following TLR stimulation in C. glabrata-infected MDMs.

Keywords:

Candida glabrata , macrophage , miR-۱۴۶a