Nickel separation from human blood samples based on amine and amide functionalized magnetic graphene oxide nano structure by dispersive sonication micro solid phase extraction

Nickel (Ni) is toxic effect on human body and must be determined in human blood samples. In this study, Ni ions separated and preconcentrated from blood samples based on magnetic Fe۳O۴-supported amine/amide-functionalized graphene oxide (Fe۳O۴@A/A-GO) nanoparticles by dispersive sonication micro solid phase extraction (DS-μ-SPE). By procedure, ۱۰ mg of Fe۳O۴@A/A-GO was dispersed in ۱۰ mL of human blood samples with sonication for ۵.۰ min and then separated from liquid phase with magnetic accessory. The Ni ions was extracted based on amine/amide covalence bonding of Fe۳O۴@A/A-GO sorbent (Ni---: NH۲). Then, the Ni ions back-extracted from Fe۳O۴@A/ A-GO in low pH with nitric acid (۰.۲ mL, ۰.۳ M) which was diluted with DW up to ۰.۵ mL and finally, was determined by ET-AAS (peak area). The LOD, linear range (LR), enrichment factor (EF) and absorption capacity (AC) were obtained ۳۵ ng L-۱, ۰.۱۵ -۷.۲ μg L-۱, ۱۹.۸ and ۱۳۱.۶ mg g-۱, respectively. The method was validated by spiking samples.

Keywords:

Nickel , Human blood , Fe۳O۴-supported Amine /Amide-functionalized graphene oxide , Dispersive sonication micro solid phase extraction