Studying the suppressing effect of mesenchymal stem cells derived from amniotic membrane on colorectal cancer
Publish place: Journal of Current Biomedical Reports، Vol: 2، Issue: 4
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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JR_JCBIOR-2-4_001
تاریخ نمایه سازی: 14 آذر 1402
Abstract:
Mesenchymal stem cell therapy is considered as a proper tool for biological activities and treatment of diseases and cancer. Here, the suppressing effect of mesenchymal stem cells derived from the amniotic membrane (AM-MSCs) on colorectal cancer (HT۲۹ cell line) was studied. MSCs were isolated from the human amniotic membrane and identity tests were performed. AM-MSC and HT۲۹ cells were co-cultured and MTT assay was used to determine proper inhibitory concentration. The apoptotic effect of AM-MSC on HT۲۹ cells was studied by Acridine orange staining. Expression of CDK۲ protein and the antioxidant effect of AM-MSCs on HT۲۹ was performed by immunocyto chemistry test. The effect of MSC on the HT۲۹ cell line migration was determined by Scratch test. The result showed that AM-MSC had proliferation-inhibitory effects and caused apoptosis of the HT۲۹ cell line. Real Time-PCR assay showed the increase of gene expression of p۵۳, Caspase۳ in apoptosis, and p۲۱ in tumor cell line treated with AM-MSC. AM-MSC arrested the cell cycle of tumor cells by reduction of CDK۲ protein. The migration of HT۲۹ and ROS expression levels was decreased. Altogether, AM-MSC may play a role in suppressing colorectal cancer HT۲۹ cell line by inhibiting angiogenesis, cell cycle, and induction of apoptosis and has antioxidant properties.
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Authors
Niloofar Mesbah
Pharmaceutical Sciences Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Nooshin Barikrow
Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Masoumeh Heshmati
Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran