Studying the suppressing effect of mesenchymal stem cells derived from amniotic membrane on colorectal cancer

Mesenchymal stem cell therapy is considered as a proper tool for biological activities and treatment of diseases and cancer. Here, the suppressing effect of mesenchymal stem cells derived from the amniotic membrane (AM-MSCs) on colorectal cancer (HT۲۹ cell line) was studied. MSCs were isolated from the human amniotic membrane and identity tests were performed. AM-MSC and HT۲۹ cells were co-cultured and MTT assay was used to determine proper inhibitory concentration. The apoptotic effect of AM-MSC on HT۲۹ cells was studied by Acridine orange staining. Expression of CDK۲ protein and the antioxidant effect of AM-MSCs on HT۲۹ was performed by immunocyto chemistry test. The effect of MSC on the HT۲۹ cell line migration was determined by Scratch test. The result showed that AM-MSC had proliferation-inhibitory effects and caused apoptosis of the HT۲۹ cell line. Real Time-PCR assay showed the increase of gene expression of p۵۳, Caspase۳ in apoptosis, and p۲۱ in tumor cell line treated with AM-MSC. AM-MSC arrested the cell cycle of tumor cells by reduction of CDK۲ protein. The migration of HT۲۹ and ROS expression levels was decreased. Altogether, AM-MSC may play a role in suppressing colorectal cancer HT۲۹ cell line by inhibiting angiogenesis, cell cycle, and induction of apoptosis and has antioxidant properties.

Keywords:

Amniotic membrane , Colorectal cancer , Co-culture , Mesenchymal stem cell