Validation of a method to assess occupational exposure to tert-buthyl mercaptan by gas chromatography- mass spectrometry (GC-MS)
Publish place: Journal of Health in the Field، Vol: 7، Issue: 1
Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JHF-7-1_006
تاریخ نمایه سازی: 14 آذر 1402
Abstract:
Background and Aims: Tert-butyl mercaptan is used as an important odorant of natural gas. Exposure to high
concentrations of this substance lead to stimulating effects in human. Occupational exposure to mercaptan is
measured by gas chromatography - flame photometer according to NIOSH ۲۵۴۲. The purpose of this study was to
develop a method for sampling and analyzing tert-butyl mercaptan using GC_MS.
Materials and Methods: In this study, tert-butyl mercaptan was analyzed using BP-۵ MS column and internal
standard of ۴-methyl thiophenol. Calibration curve was developed and evaluated in the range of ۰.۴۵۱۰- ppm.
Repeatability of method was presented by inter- day and intra-day assay based on the coefficient of variation
(CV). Recovery of tert-butyl mercaptan was reported as the accuracy of method. All procedures of this study were
approved by Shahid beheshti University of medical Science Institutional Review Board.
Results: Retention time of tert-butyl mercaptan and ۴-methylthiophenol were determined as ۴.۰۵ and ۸.۰۱ min,
respectively. Calibration curve was evaluated by the regression of ۰.۹۹. The mean recovery percent was ۹۸.۶۸ ± ۶.۶.
Intra-day and inter-day coefficient of variance for standard concentrations was ranged between ۰.۴۶-۷.۲ percent.
The limit of detection and limit of quantitation were presented ۰.۰۳۷ and ۱.۸۴ μg/sample, respectively.
Conclusion: GC-MS method by validated parameters was suggested as a suitable alternative for NIOSH method.
Authors
Rezvan Zendehdel
Associate Professor, Department of Occupational Health Engineering and Safety, School of Public Health and Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Seyed Mohammad Sadat
MSc, Department of Occupational Health Engineering and Safety, School of Public Health and Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Farzad Kobarfard
Professor, Department of Medicinal Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Samira Eslamizad
Assisstant Professor, Food Safety Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran