Selection of antibacterial peptides against methicillin-resistant Staphylococcus aureus by bioinformatics and laboratory integration methods

Common hospital infections caused by methicillin-resistant Staphylococcus aureus (MRSA) bacteria are one of the most common causes of death in hospitalized people. MRSA is resistant to antibiotics and common treatments. However, so far, two drugs designed based on Anti-Microbial Peptides (AMP) have been e↵ective in controlling and eliminating them. Antimicrobial peptides are immune molecules with a small and simple structure and effective function, which are often found in insects and plants, although they have also been observed and recorded in the body tissues of living organisms, for example, In mammals, AMP is found mainly in the granules of neutrophils and in the secretions of epithelial cells that cover the skin and mucosal surfaces. AMPs can be effective on destroying bacteria, viruses, fungi, parasite or even tumors. But using AMP as drug can have potential side e↵ects. It’s a new and unknown field of research. The aim of this research was to find a safe and effective AMP against MRSA. In this research, all peptides effective on MRSA were found from the AMP database and their characteristics were noted. Then their antigenicity, allergenicity and toxicity were investigated. In the second step, homology with human proteins and peptides was checked and peptides that were non-allergenic, non-antigenic, non-toxic and had high homology with human proteins were selected and their interactions with seven bacterial surface proteins and two bacterial internal proteins were investigated. Became. Among them, two peptides were selected as the main candidates for synthesis and future tests, one of which was selected with a shorter amino acid chain length

Keywords:

MRSA , AMP , Common hospital infections , Anti-Microbial Peptides