Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms
Publish place: Archives of Razi Institute journal، Vol: 78، Issue: 2
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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JR_ARCHRAZI-78-2_011
تاریخ نمایه سازی: 6 دی 1402
Abstract:
Antioxidant and antibacterial chemicals are key sources in medicinal plants. Alkaloids, phenolics, steroids, terpenes, flavonoids, terpenes, and volatile oils are a few of these plants' secondary metabolites. Phytochemicals, particularly the secondary metabolites produced by plants, are important for human nutrition, well-being, illness prevention, and antibacterial properties. This study aimed to ascertain the aqueous broccoli extract's chemical makeup. The phytochemical molecule that the GC-MS technique identified. The DPPH assay, which is appropriate for regular plant material screening, was performed to assess the antioxidant capacities of broccoli extract (in vitro). Subsequently looks at how well they perform against different Gram-positive and Gram-negative harmful microorganisms. GC-MS analysis of Broccoli extract revealed the existence of the۹-Octadecenamide, [C۱۸H۳۵O], Hexadecane [c۱۶h۳۴] and ۲, ۲, ۲-trifluoroethyl ۲-methyltetrahydro-۵-oxo-۳- furancarboxylate [C۲۳H۳۳NO۶]. There were significant changes in the extract's ascorbic acid-free radical scavenging activity at ۲۰۰, ۱۰۰, and ۲۵ g/ml (P ۰.۰۵), and the activity was dose-dependent. The effectiveness of aqueous broccoli extract as a powerful, broad-spectrum antibacterial agent against tested bacteria is demonstrated by an increase in the diameter of the inhibition zone, which increases in direct proportion to the concentration of the extract and even surpasses the activity of some antibiotics. An appropriate concentration of aqueous broccoli extract strongly inhibits microbial and antioxidant growth, especially when treating external infections without any danger against resistant bacterial isolates; it is strongly advised to use aqueous broccoli extract as a cost-effective alternative antibacterial and antioxidant agent.
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Authors
Z Al Talebi
Chemistry Department, College of Science, University of Babylon, Babylon, Iraq
M. M Karhib
Department of Medical Laboratory Techniques, Al-Mustaqbal University College, ۵۱۰۰۱ Hillah, Babylon, Iraq
M. M Taki
Nanotechnology and Advanced Materials Research Centre, University of Technology, Baghdad, Iraq
E Salaam Abood
Medical Physics Department, Hilla University College, Babylon, Iraq
H. A Mubarak
Department of Chemical Engineering, College of Engineering, University of Babylon, Babylon, Iraq
C Sahar Ahmed Ibrahim
Babil Teaching Hospital For Maternity and Children, Babylon, Iraq
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