Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields

Pullorum disease (PD) is one of the most common diseases in the world, with devastating consequences. In the chicken sector, there have been financial losses. It is brought on by Salmonella enteric subspecies serovar Gallinarum biovar pullorum; definitive detection requires culture followed by biochemistry analysis and serotyping. This study aimed to verify the presence of bacteria by culture, biochemical characterization, PCR assay, and sequencing. One hundred samples were collected from ۱۲ broiler chicken flocks of different ages for ۸districts of Baghdad province, including cloacal swabs (۶۵), visceral organs (۱۵), and dropping (۲۰). Salmonella colonies were identified by selective culture broth and agar with biochemical description for ۷۵% of the total samples, with a higher incidence in visceral organs than dropping and cloacal swabs. ،The Sequencing and phylogenetic tree analysis of ۱۶S rRNA gene for representative Salmonella isolates. The presence of Salmonella pullorum isolates in global genetic strains; was revealed a matching NCBI isolates similarity of ۹۹.۰۲% with (MF۴۴۵۱۲۴.۱) and ۹۸% with (MH۳۵۲۱۶۴.۱), respectively. In the current state of molecular and genetic research, phlyogentic research announced the real presence of Salmonella pullorum in Baghdadprovince's broiler chicken, also showing the phylogentic characteristics and links to some global isolates. The detection of Salmonella pullorum in broiler flocks of the current study extent of health risks to other uninfected birds present in the free range.

Keywords:

Salmonella pullorum , biochemical tests , PCR , Phylogenetic analysis