Pharmacognostic Profile and Screening of Anti-Proliferative Potential of Methanolic Extract of Tripterygium wilfordii Plant on WRL-۶۸ Cell Line and Function of Polycystin‐۱

Tripterygium wilfordii is a medicinal plant that plays a crucial role in health care programs, especially in developing countries, and had anti-tumor, anti-inflammatory, anti-fertility, anti-bacterial, and other therapeutic effects. This study was designed to determine the anti-proliferative effects of methanolic extract of T. wilfordii on the WRL-۶۸ cell line and the function of polycystin‐۱ (PC-۱). The half-maximal inhibitory concentration (IC۵۰) values were recorded in WRL-۶۸ and AsPC-۱ cell lines as ۱۹۳ µg/ml and ۱۴۹.۲ µg/ml, respectively, at ۲-۲.۵۵ and ۲-۲.۲ µg/ml methanolic plant concentrations. The maximum cytotoxic activities of the extract on the growth inhibition of WRL-۶۸ and AsPC-۱ were generally observed at ۹۷.۶۴% and ۹۵.۹۴% at extract concentrations of ۵۰ µg/ml and ۲۵ µg/ml, respectively. The pharmacognostic profile of T. wilfordii extract was found to be alkaloids, tannins, terpenoides, flavonoids, glycosides, and phenols. The extracts of T. wilfordii were tested through gas chromatography-mass spectrometry showing four peaks representing mostly of ۳-Oxobutanol; ethyl acetate; acetic acid ethyl ester; chlorbromuron; ۱-(methylthio)-, (E)-; n-Hexadecanoic acid; tetradecanoic acid; and ۹-Octadecenoic acid. Therefore, the results of this study revealed that the methanolic extract of T. wilfordii was more potential in inducing anti-proliferative activity of WRL-۶۸ and AsPC-۱ human cell lines than the control. In addition, the current study was the first study that reported the anti-proliferative potential of T. wilfordii in the treatment of human embryonic liver WRL-۶۸ cancer cells.

Keywords:

Anti-proliferative , GC-MS , MTT , Pharmacognostic profile , Tripterygium wilfordii plant