Molecular Detection of Hepatozoon canis in Dogs of Ardabil Province, Northwest of Iran
Publish place: Archives of Razi Institute journal، Vol: 72، Issue: 3
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_ARCHRAZI-72-3_007
تاریخ نمایه سازی: 6 دی 1402
Abstract:
Hepatozoon species are protozoan parasites that infect some animals such as birds, reptiles, amphibians, and carnivores. Previous studies performed on canine hepatozoonosis in Iran have never used molecular techniques for diagnosis of this disease. The main objective of the present study was to detect Hepatozoon canis in the blood of dogs using polymerase chain reaction (PCR) method and sequencing. A total of ۱۰۴ blood samples were collected from dogs of Meshginshahr County (Ardabil Province), and DNA was extracted from blood samples by dint of DNG-plus Extraction Kit. Then, ۱۸S rRNA gene was amplified by using the conventional PCR methods. PCR products yielded an amplicon of the approximate length of ۸۹۷ bp for all the positive samples. Twenty-four out of the ۱۰۴ (۲۳.۰۷%) samples were found to be positive for H. canis. This rate of infection is relatively high among dogs in Ardabil Province. Sequence analysis confirmed the molecular identity of ۹۹% of the samples by comparison with GenBank profiles. This is the first report of molecular detection of H. canis from Iran.
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Authors
A. Dalimi
Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
F. Jameie
Faculty of Medical Sciences, Tarbiat Modares University
A. Mohammadiha
Faculty of Medical Sciences, Tarbiat Modares University
M. Barati
Infectious diseases Research Center, Aja University of Medical Sciences
S. Molaei
Faculty of Medical Sciences, Tarbiat Modares University
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