Molecular epidemiology of FMDV in Isfahan province of Iran (۲۰۰۶-۲۰۰۹)

It is about ۵۰ years that FMD affected the ruminants of Isfahan. Last outbreaks of FMD were happened at ۲۰۰۵ even vaccinated animals, so in current work using RT-PCR, sequencing and regression "r" values, the isolated strains in Isfahan were identified. The aim of this study was molecular epidemiology of FMDV in Isfahan province as the central part of Iran in ۲۰۰۶-۲۰۰۹. According to the result , a highly pathogen A۰۵ strain was isolated from west (Najafabad city) about ۲ months after the entrance of this virus to Iran through the west and north west margins toward central part and then distributed around ۱۰ cities of Isfahan province. Here it is obvious that the A۰۵ strain of Isfahan just showed ۱% difference with A۰۵IR (vaccine strain), in which for A۲۲ were ۶۵ %. Also based on the alignment of ۶۰۰ bp of ۳΄ end of the VP۱ sequences of isolated type O comparing with representative of type O Shabestar vaccine strain and the other provinces of Iran, the Isfahan O isolate was ۳% distinct from O shabestar vaccine strain. In a random "r" value detection of west isolate strain (A /Najafabad/Isfahan/Iran/ ۰۵) against A۸۷IR were ۰.۳۵ and against A۰۵IR were ۰.۷۳; For O strain, randomly "r" value of center isolate (O/Isfahan/Isfahan/Iran) obtained against Iranian O vaccine strain (O Shabestar) were ۰.۷۶ and with O ۹۶۷ (Panasia) were ۰.۸۸. Regarding to the conclusion, the FMD lived vaccine for Isfahan was improved with A۰۵/Ir FMDV by Razi Vaccine and Serum Research Institute (RVSRI).

Keywords:

Foot , and , Mouth disease , Molecular epidemiology , Isfahan , "r" value