Biochemical and Histopathological study of Mesobuthus eupeus scorpion venom in the experimental rabbits

In tropical and subtropical countries, envenomation by scorpions (so-called scorpionism) represents a serious public health problem. In the present study, the toxic effects of mice LD۵۰ injections of Mesobuthus eupeus (Me) venom on the kidney and liver of anesthetized rabbits were investigated. Six rabbits were selected and ALT, AST, BUN and creatinine were measured at ۰, ۱ and ۳ hours after envenomation and histopathological studies were carried out postmortem. All the animals showed signs and symptoms of envenomation within ۳۰-۴۰ minutes and died ۳ to ۳.۵ hours after venom injection. Histopathological examinations revealed glumerolar congestion, dilated vessels of interstitium and focal interstitial congestion in the kidney and focal hemorrhage, central vein congestion, congested vessels in portal areas and dilated sinusoids in the liver at ۳ to ۳.۵ hrs following venom injection. In addition, biochemical analyses indicated significant rise in the levels of ALT and creatinine following Mesobuthus eupeus envenomation in animals at ۳ hrs. However no significant changes were observed at ۱ hr. In conclusion, scorpion (Mesobuthus eupeus) venom leads to damage in vital organs such as liver and kidney.

Keywords:

Mesobuthus eupeus , Rabbit , envenomation , histopathological changes , ALT , creatinine