Detection of Paenibacillus larvae larvae spores in honey and diseased larvae Samples by culture and PCR

American foulbrood (AFB) is the most serious brood disease of the honey bee. Traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. The aim of this study is the detection of Paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and PCR. Therefore ۵۴ samples of diseased larvae and ۳۶ honey samples, were diluted with an equal volume of distilled water and centrifuged, then the pellet was used for bacterial culture, DNA extraction and PCR. PCR products were electrophoresed on ۰.۸ % agarose gel. Five of ۵۴ (۹.۳ %) larvae samples and ۵ of ۳۶ (۱۳.۹ %) honey samples were positive for Paenibacillus larvae larvae by culture and PCR. As a result, screening of honey and larvae samples by PCR method proved to be a reliable, fast and useful method on regional and national scale for monitoring, controlling, and using preventive measures before the occurrence of American foulbrood damages.

Keywords:

Hony bee , Paenibacillus larvae , American Foulbrood(AFB) , Brood disease , PCR