Detection of Mycoplasma synoviae infection in broiler breeder farms of Tehran province using PCR and culture methods

Mycoplasma synoviae (MS) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the Iranian chicken industry especially breeder farms. The aim of this study was to use the MS specific PCR and culture methods in order to detect of M. synoviae from breeder farms where located in Tehran province. A total of ۴۷۵ samples including choanal cleft, trachea, ovary and /or joint cavities from ۲۳ broiler breeder farms of Tehran area were collected. Samples were cultured in PPLO broth media supplemented for MS isolation. The bacteria DNAs were extracted by phenol/chloroform method. Specific published primers amplify a ۲۰۷ bp region of the ۱۶S rRNA gene of MS were used for PCR method. Out of ۴۷۵ samples, ۱۴۶ cultures were shown positive and typical Mycoplasma colonies, ۸۵ samples were also identified MS based on agglutination test with specific MS antiserum and the PCR method. A total of ۱۲۲ samples, a band with ۲۰۷ bp was shown as MS specific PCR product in electrophoresis. In addition to these ۸۵ samples that were positives in both culture and PCR, ۳۷ samples that had not grown in Mycoplasma media were positive in MS specific PCR. A total of ۲۹۲ samples were negatives in both culture and PCR methods. ۱۲۲ positive samples out of ۴۷۵ samples (۲۵.۷%) were belonged to ۷ breeder farms (۳۰.۴%). On conclusions, the MS infection of broiler breeder farms of Tehran area was confirmed truly. From the results, as the PCR method reduces the time consuming, an effectiveness and efficient for detection of M. synoviae infection of chicken breeder. It is then suggested that the PCR method could be an alternative method for culturing.

Keywords:

Mycoplasma synoviae , broiler breeder , PCR , Culture , Tehran