Quantification of thymol content in different extracts of Zataria multiflora by HPLC method
Publish place: Pharmaceutical and Biomedical Research، Vol: 2، Issue: 1
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
View: 100
This Paper With 6 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_PBRE-2-1_002
تاریخ نمایه سازی: 10 دی 1402
Abstract:
Zataria multiflora Boiss is used in traditional folk remedies as antiseptic, analgesic, carminative, anthelmintic medication. The main components of Z. multiflora are phenolic compounds such as thymol and carvacrol. The aim of this study was developed a simple and rapid method for determination of the thymol in different extracts of Z. multiflora by HPLC method. The dried aerial parts of Z. multiflora in the flowering stage was extracted with ethanol ۳۴%, ۴۲% and ۷۰% for ۴۸ hours. Several mobile phase systems were applied for development and separation of thymol and carvacrol that have close retention time in HPLC system. The peaks of thymol and carvacrol are successfully separated in acetonitrile-water-acetic acid mobile phase. Thymol and carvacrol were separated with a retention times ۱۰.۴ and ۹.۸ minutes respectively, in an isocratic solvent system with HPLC. Thymol content was ۲.۷ ± ۰.۰۶, ۳.۷ ± ۰.۰۷ and ۶.۰ ± ۰.۱۱ mg/g, in ethanol at concentration of ۳۴%, ۴۲% and ۷۰%, respectively. Thymol content in different hydroalcoholic extract of Z. multiflora is dependent to ethanol concentration in extraction solvent.
Keywords:
Authors
Azar Aghamohammadi
Traditional and Complementary Medicine Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Mohammad Azadbakht
Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Seyed Jalal Hosseinimehr
Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :